Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin     

Suzan Pantaroto de Vasconcellos  Elaine Crespim  Georgiana Feitosa da Cruz  Diego Barbosa Senatore  Karen Christina Marques Simioni  Eugênio Vaz dos Santos Neto  Anita Jocelyne Marsaioli  Valéria Maia de Oliveira 《Organic Geochemistry》2009,40(5):574-588

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.  相似文献   

Another Year of Record Heat for the Oceans     

Lijing CHENG  John ABRAHAM  Kevin E.TRENBERTH  John FASULLO  Tim BOYER  Michael E.MANN  Jiang ZHU  Fan WANG  Ricardo LOCARNINI  Yuanlong LI  Bin ZHANG  Fujiang YU  Liying WAN  Xingrong CHEN  Licheng Feng  Xiangzhou SONG  Yulong LIU  Franco RESEGHETTI  Simona SIMONCELLI  Viktor GOURETSKI  Gengxin CHEN  Alexey MISHONOV  Jim REAGAN  Guancheng LI 《大气科学进展》2023,40(6):963-974

Changes in ocean heat content(OHC), salinity, and stratification provide critical indicators for changes in Earth’s energy and water cycles. These cycles have been profoundly altered due to the emission of greenhouse gasses and other anthropogenic substances by human activities, driving pervasive changes in Earth’s climate system. In 2022, the world’s oceans, as given by OHC, were again the hottest in the historical record and exceeded the previous 2021 record maximum.According to IAP/CAS data, ...  相似文献   

Correction to: Trace ambient levels of particulate mercury and its sources at a rural site near Delhi     

Anita Kumari  Umesh Kulshrestha 《Journal of Atmospheric Chemistry》2018,75(4):357-357

  相似文献   

Divergent trends in ecosystem services under different climate-management futures in a fire-prone forest landscape     

Joshua S. Halofsky  Jessica E. Halofsky  Miles A. Hemstrom  Anita T. Morzillo  Xiaoping Zhou  Daniel C. Donato 《Climatic change》2017,142(1-2):83-95

While ecosystem services and climate change are often examined independently, quantitative assessments integrating these fields are needed to inform future land management decisions. Using climate-informed state-and-transition simulations, we examined projected trends and tradeoffs for a suite of ecosystem services under four climate change scenarios and two management scenarios (active management emphasizing fuel treatments and no management other than fire suppression) in a fire-prone landscape of dry and moist mixed-conifer forests in central Oregon, USA. Focal ecosystem services included fire potential (regulating service), timber volume (provisioning service), and potential wildlife habitat (supporting service). Projections without climate change suggested active management in dry mixed-conifer forests would create more open forest structures, reduce crown fire potential, and maintain timber stocks, while in moist mixed-conifer forests, active management would reduce crown fire potential but at the expense of timber stocks. When climate change was considered, however, trends in most ecosystem services changed substantially, with large increases in wildfire area predominating broad-scale trends in outputs, regardless of management approach (e.g., strong declines in timber stocks and habitat for closed-forest wildlife species). Active management still had an influence under a changing climate, but as a moderator of the strong climate-driven trends rather than being a principal driver of ecosystem service outputs. These results suggest projections of future ecosystem services that do not consider climate change may result in unrealistic expectations of benefits.  相似文献   

中等传输距离下的无线功率传输链路第1部分:实现工作状态最优化     

Alessio De ANGELIS  Marco DIONIGI  Paolo CARBONE  Mauro MONGIARDO  车文荃  王清华  Franco MASTRI  Giuseppina MONTI 《南京气象学院学报》2017,9(1):64-72

中程无线功率传输（WPT）可以采用几种不同的方式实现,如通过电感或电容耦合、谐振或非谐振网络实现.本文主要研究了通过感应耦合谐振器实现的WPT链路,而且只着重研究了利用2个谐振器的链路（直接链路）并工作在主谐振频率下的情况.研究结果表明,当工作在主谐振频率下,可以根据网络参数来对传输效率或负载功率进行优化.  相似文献   

Influence of lunar phases,winds and seasonality on the stranding of mesopelagic fish in the Strait of Messina (Central Mediterranean Sea)          下载免费PDF全文

Pietro Battaglia  Giovanni Ammendolia  Mauro Cavallaro  Pierpaolo Consoli  Valentina Esposito  Danilo Malara  Ignazio Rao  Teresa Romeo  Franco Andaloro 《Marine Ecology》2017,38(5)

The shore stranding of mesopelagic fauna is a recurrent phenomenon in the Strait of Messina (Central Mediterranean Sea). The aim of this paper is to test the influence of lunar phases, winds and seasons upon the frequency of occurrence of strandings of mesopelagic fish. Species abundance in relation to these factors was quantified for the first time. Specimens were collected stranded on the shore along the Sicilian coast of the Strait of Messina between 2008 and 2016. Overall 32 species belonging to seven families (Gonostomatidae, Microstomatidae, Myctophidae, Paralepididae, Phosichthyidae, Sternoptychidae, Stomiidae) were found stranded. Myctophidae was the family including the highest number of species (16), whereas Gonostomatidae was the most abundant in terms of total number of individuals (47.2%), mainly thanks to the species Cyclothone braueri. The moon, which influences the strength of currents (highest during full and new moon phases) and irradiance (higher in some lunar phases, such as the full moon), affected the abundance of stranded mesopelagic fish in the study area. The highest number of stranding events was recorded during the new moon: 34.6% of the total relative abundance of stranded mesopelagic fish. Wind blowing from the sea towards the coastline (southeasterly and easterly winds) created the best conditions for strandings. The highest abundance of stranded specimens was recorded during the winter season.  相似文献   

Land Surface Temperature Patterns in the Urban Agglomeration of Krakow (Poland) Derived from Landsat-7/ETM+ Data   总被引：1，自引：0，他引：1  

Jakub P. Walawender  Mariusz Szymanowski  Monika J. Hajto  Anita Bokwa 《Pure and Applied Geophysics》2014,171(6):913-940

  相似文献   

河南铁炉坪银矿床的地质和D-O-C同位素体系及成因   总被引：2，自引：0，他引：2  

陈衍景  Franco PIRA JNO  隋颖慧 《地质学报》2005,79(1):144

铁炉坪银矿产于熊耳地体高级变质基底内的 NE向断裂带中 ,是最新发现的一个重要的中生代造山型 Ag- Pb和Au矿带的一部分。成矿过程包括早 (E)、中 (M)、晚 (L ) 3个阶段 ,分别形成石英 -黄铁矿组合 (E)、多金属硫化物组合(M)和碳酸盐脉 (L)。早阶段流体的 δD=- 90‰ ,δ1 3CCO  相似文献   

Distribution of trace elements in filtered and non filtered aqueous fractions: Insights from rivers and streams of Sardinia (Italy)     

Rosa Cidu  Franco Frau 《Applied Geochemistry》2009

The distribution of several trace elements in different aqueous fractions has been studied in running waters from Sardinia (Italy). Trace elements and major components were determined in water samples collected at high- and low-discharge from rivers (90 samples) and streams (70 samples). At selected sites, total (non filtered samples) and dissolved (0.4 μm and 0.015 μm pore-size filtered samples) amounts of trace elements were determined, and the composition of the solid matter retained on the filters was investigated for estimating the eventual interrelationship. The elements B, Li, Rb, Sr, Ba, As, Sb, Mo, Tl and U in the studied waters showed small differences between total and dissolved amounts; dissolved concentrations were higher under low flow conditions, when the contribution of rainwater to the rivers was minimum; their concentrations were often correlated with total dissolved solids (TDS), and appeared to be related to the intensity of water-rock interaction processes. The elements Al, Fe, Mn, Pb, Zn, Cd, Cu, Co, Ni, Cs, Y, REE and Th were not related to TDS and/or major ions; they showed higher concentrations under high flow conditions; marked differences occurred between total and dissolved amounts; much lower concentrations were generally observed in the water filtered through 0.015 μm than in the water filtered through 0.4 μm, especially when sampling was carried out after heavy rain events that enhanced the load of solid matter in the water. These observations indicate an aqueous transport via sorption processes on very fine particles, such as Fe-oxide/hydroxide and clay mineral particles, which have been inferred by SEM-EDX analyses of the matter retained on the filters.  相似文献   

Low-pH waters discharging from submarine vents at Panarea Island (Aeolian Islands,southern Italy) after the 2002 gas blast: Origin of hydrothermal fluids and implications for volcanic surveillance     

Franco Tassi  Bruno Capaccioni  Giorgio Caramanna  Daniele Cinti  Giordano Montegrossi  Luca Pizzino  Fedora Quattrocchi  Orlando Vaselli 《Applied Geochemistry》2009

A geochemical survey of thermal waters collected from submarine vents at Panarea Island (Aeolian Islands, southern Italy) was carried out from December 2002 to March 2007, in order to investigate (i) the geochemical processes controlling the chemical composition of the hydrothermal fluids and (ii) the possible relations between the chemical features of the hydrothermal reservoir and the activity of the magmatic system. Compositional data of the thermal water samples were integrated in a hydrological conceptual model, which describes the formation of the vent fluid by mixing of seawater, seawater concentrated by boiling, and a deep, highly-saline end-member, whose composition is regulated by water-rock interactions at relatively high temperature and shows clear clues of magmatic-related inputs. The chemical composition of concentrated seawater was assumed to be represented by that of the water sample having the highest Mg content. The composition of the deep end-member was instead calculated by extrapolation assuming a zero-Mg end-member. The Na–K–Ca geothermometer, when applied to the thermal end-member composition, indicated an equilibrium temperature of approximately 300 °C, a temperature in agreement with the results obtained by gas-geothermometry.  相似文献